Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean
Publication in refereed journal



摘要During seed development and maturation, large amounts of storage proteins are synthesized and deposited in protein storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via immunogold electron microscopy in developing mung bean cotyledons. It is demonstrated that non-aggregated 8S globulins are present in multivesicular bodies (MVBs) in early stages of cotyledon development where neither dense vesicles (DVs) nor a PSV were recognizable. However, at later stages of cotyledon development, condensed globulins were visible in both DVs and distinct MVBs with a novel form of partitioning, with the internal vesicles being pushed to one sector of this organelle. These distinct MVBs were no longer sensitive to wortmannin. This study thus indicates a possible role for MVBs in transporting storage proteins to PSVs during the early stage of seed development prior to the involvement of DVs. In addition, wortmannin treatment is shown to induce DVs to form aggregates and to fuse with the plasma membrane.
著者Wang JQ, Tse YC, Hinz G, Robinson DG, Jiang LW
期刊名稱Journal of Experimental Botany
出版社Oxford University Press (OUP): Policy B - Oxford Open Option A
頁次1367 - 1380
關鍵詞Dense vesicle; endosome; multivesicular body; protein storage vacuole; storage pre-vacuolar compartment; storage protein; wortmannin
Web of Science 學科類別Plant Sciences; PLANT SCIENCES

上次更新時間 2020-17-10 於 00:36