Entrapment of a Trigonopsis variabilis D-amino acid oxidase variant F54Y for oxidative deamination of cephalosporin C
Publication in refereed journal

香港中文大學研究人員

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摘要Trigonopsis variabilis D-amino acid oxidase (TvDAAO) is an enzyme used in the industrial bioconversion of cephalosporin C (CPC) into 7-aminocephalosporanic acid, a crucial biosynthetic nucleus for a wide spectrum of semi-synthetic cephem antibiotics. Using homology modeling and site-directed mutagenesis, we have previously shown that the TvDAAO variant F54Y possesses improved catalytic activity and thermostability. To further explore its industrial application, the conditions for immobilization of the enzyme were examined in the present investigation. The results showed that entrapment in a calcium alginate (Ca-alginate) matrix using 2% alginate, 500mM CaCl2, and 15 min stabilization appeared to be optimal for the immobilization of F54Y. The entrapped enzyme allowed complete CPC conversion. The entrapped enzyme also showed good operational stability and retained at least 90% of its original activity after 20 reaction cycles. To conclude, the entrapment of F54Y in Ca-alginate appeared to be a simple and efficient biocatalysis system with potential application in the antibiotics industry.
著者Wong KS, Fong WP, Tsang PWK
期刊名稱Engineering in Life Sciences
出版年份2011
月份10
日期1
卷號11
期次5
出版社Wiley: 12 months
頁次491 - 495
國際標準期刊號1618-0240
語言英式英語
關鍵詞Alginate; D-Amino acid oxidase; Entrapment
Web of Science 學科類別Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY

上次更新時間 2020-27-10 於 00:52