Efficacy and Durability in Direct Labeling of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles with Organosilica, Dextran, and PEG Coatings
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摘要We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6-7 nm. Together with their coatings, the overall sizes are 10-15 nm for all SPIO@SiO(2), SPIO@dextran, and SPIO@PEG nanoparticles. These nanoparticles were investigated for their efficacies to be uptaken by rabbit bone marrow-derived MSCs without any transfecting agent. Experimentally, both SPIO@SiO(2) and SPIO@PEG nanoparticles could be successfully uptaken by MSCs while the SPIO@dextran nanoparticles demonstrated limited labeling efficiency. The labeling durability of SPIO@SiO(2) and SPIO@PEG nanoparticles in MSCs after three weeks of culture were compared by Prussian blue staining tests. SPIO@SiO(2) nanoparticles demonstrated more blue staining than SPIO@PEG nanoparticles, rendering them better materials for MSCs labeling by direct uptake when durable intracellullar retention of SPIO is desired.
著者Wang YXJ, Quercy-Jouvet T, Wang HH, Li AW, Chak CP, Xuan SH, Shi L, Wang DF, Lee SF, Leung PC, Lau CBS, Fung KP, Leung KCF
期刊名稱Materials
詳細描述2011; doi:10.3390/ma4040703.
出版年份2011
月份4
日期1
卷號4
期次4
出版社MDPI
頁次703 - 715
國際標準期刊號1996-1944
語言英式英語
關鍵詞cell labeling; magnetic nanoparticle; surface modification
Web of Science 學科類別Materials Science; Materials Science, Multidisciplinary; MATERIALS SCIENCE, MULTIDISCIPLINARY

上次更新時間 2020-28-11 於 23:48