Formulation of sustained-release microspheres of granulocyte macrophage colony stimulating factor by freezing-induced phase separation with dextran and encapsulation with blended polymers
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AbstractThis study aimed to assess the potential merits of formulating sustained-release microspheres of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) via freezing-induced phase separation (FIPS) of the protein with dextran followed by encapsulation with binary mixture of poly(lactic-co-glycolic acid) (PLGA) 2A (MW similar to 12K) and 3A (MW similar to 47K) or of PLGA2A and polylactic acid (PLA; MW similar to 83K). The formulated dextran particles and microspheres were characterized in vitro for loading, aggregation, bioactivity and release behavior of the protein where appropriate. rhGM-CSF retained about 60% of bioactivity with no significant aggregation after each formulation step. Encapsulation of protein-loaded dextran particles attained only 80% with the PLGA2A and PLGA3A blend, but 100% with the PLGA2A and PLA mixture. The former formulation exhibited a triphasic in-vitro release profile typical of PLGA microspheres while the latter revealed a much lower initial burst followed by a steady and complete release of rhGM-CSF with preserved bioactivity over a 15-day period.
All Author(s) ListZhang XR, Zheng Y, Jin T, Chow AHL
Journal nameJournal of Microencapsulation
Year2011
Month1
Day1
Volume Number28
Issue Number8
PublisherTaylor & Francis: STM, Behavioural Science and Public Health Titles
Pages743 - 751
ISSN0265-2048
eISSN1464-5246
LanguagesEnglish-United Kingdom
Keywordsdextran-stabilized protein; freezing-induced phase separation; PLGA and PLA polymer blends; rhGM-CSF microspheres
Web of Science Subject CategoriesChemistry; Chemistry, Applied; CHEMISTRY, APPLIED; Engineering; Engineering, Chemical; ENGINEERING, CHEMICAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-24-10 at 01:40