Two genes, rif15 and rif16, of the rifamycin biosynthetic gene cluster in Amycolatopsis mediterranei likely encode a transketolase and a P450 monooxygenase, respectively, both essential for the conversion of rifamycin SV into B
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香港中文大學研究人員

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摘要Amycolatopsis mediterranei produces an important antibiotic rifamycin, the biosynthesis of which involves many unusual modifications. Previous work suggested a putative P450 enzyme encoded by rif16 within the rifamycin biosynthetic gene cluster (rif) was required for the conversion of the intermediate rifamycin SV into the end product rifamycin B. In this study, we genetically proved that a putative transketolase encoded by rif15 is another essential enzyme for this conversion. Expression of merely rif15 and rif16 in a rif cluster null mutant of A. mediterranei U32 was able to convert rifamycin SV into B. However, this Rif15- and Rif16-mediated conversion was only detected in intact cells of A. meidterranei, but not in Streptomyce coelicolor or Mycobacterium smegmatis, suggesting that yet-characterized gene(s) in A. mediterranei other than those encoded by the rif cluster should be involved in this process.
著者Yuan H, Zhao W, Zhong Y, Wang J, Qin ZJ, Ding XM, Zhao GP
期刊名稱Acta Biochimica et Biophysica Sinica
出版年份2011
月份12
日期1
卷號43
期次12
出版社Oxford University Press (OUP): Policy B - Oxford Open Option D / Wiley: No OnlineOpen
頁次948 - 956
國際標準期刊號1672-9145
電子國際標準期刊號1745-7270
語言英式英語
關鍵詞Amycolatopsis mediterranei; P450; rifamycin; transketolase
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS

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