Lentivirus-Mediated RNA Interference Targeting Bax Inhibitor-1 Suppresses Ex Vivo Cell Proliferation and In Vivo Tumor Growth of Nasopharyngeal Carcinoma
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摘要Bax inhibitor-1 (Bi-1), an anti-apoptotic protein that belongs to the Bcl-2 family, plays an important role in the mitochondrial apoptosis pathway to suppress Bax-induced apoptosis. in several human cancers, including nasopharyngeal carcinoma, its expression was found to be increased; however, up-regulated expression of this protein has been linked to increased cell proliferations. In this study, we down-regulated the gene expression of Bit in nasopharyngeal carcinoma cells by using a lentivirus transfection system packed with short hairpin RNA targeting Big and used an in vivo model to assess its efficacy as a target in human gene therapy. The data indicated that human malignant nasopharyngeal carcinoma cells, CNE-1 and SUNE-1, transfected with lentiviral short hairpin RNA targeting Bi-1 grew more slowly and showed a higher degree of apoptosis. Moreover, the tumorigenicity of CNE-1 was significantly suppressed when inoculated mice were intratumorically injected with the same vector. Taken together, these data lead us to conclude that Bi-1 plays a crucial role in CNE-1 tumorigenesis and that Bi-1 may be a novel therapeutic target for nasopharyngeal carcinoma
著者Li XY, Lai YK, Zhang JF, Luo HQ, Zhang MH, Zhou KY, Kung HF
期刊名稱Human Gene Therapy
出版年份2011
月份10
日期1
卷號22
期次10
出版社Mary Ann Liebert
頁次1201 - 1208
國際標準期刊號1043-0342
語言英式英語
Web of Science 學科類別Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Genetics & Heredity; GENETICS & HEREDITY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine

上次更新時間 2020-13-10 於 00:37