Lentivirus-Mediated RNA Interference Targeting Bax Inhibitor-1 Suppresses Ex Vivo Cell Proliferation and In Vivo Tumor Growth of Nasopharyngeal Carcinoma
Publication in refereed journal


摘要Bax inhibitor-1 (Bi-1), an anti-apoptotic protein that belongs to the Bcl-2 family, plays an important role in the mitochondrial apoptosis pathway to suppress Bax-induced apoptosis. in several human cancers, including nasopharyngeal carcinoma, its expression was found to be increased; however, up-regulated expression of this protein has been linked to increased cell proliferations. In this study, we down-regulated the gene expression of Bit in nasopharyngeal carcinoma cells by using a lentivirus transfection system packed with short hairpin RNA targeting Big and used an in vivo model to assess its efficacy as a target in human gene therapy. The data indicated that human malignant nasopharyngeal carcinoma cells, CNE-1 and SUNE-1, transfected with lentiviral short hairpin RNA targeting Bi-1 grew more slowly and showed a higher degree of apoptosis. Moreover, the tumorigenicity of CNE-1 was significantly suppressed when inoculated mice were intratumorically injected with the same vector. Taken together, these data lead us to conclude that Bi-1 plays a crucial role in CNE-1 tumorigenesis and that Bi-1 may be a novel therapeutic target for nasopharyngeal carcinoma
著者Li XY, Lai YK, Zhang JF, Luo HQ, Zhang MH, Zhou KY, Kung HF
期刊名稱Human Gene Therapy
出版社Mary Ann Liebert
頁次1201 - 1208
Web of Science 學科類別Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Genetics & Heredity; GENETICS & HEREDITY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine

上次更新時間 2022-11-01 於 23:30