Application of FINS and multiplex PCR for detecting genuine abalone products
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AbstractAbalones rank first on the list of the four treasures from the sea in Chinese cuisine. It is regarded as a tonic for yin-enrichment and nourishing the lungs and liver. Hong Kong is a major entrepot of abalones, importing 48% of the global production volume. Rapid detection of substitutes in abalone products is essential for safeguarding consumer interests and ensuring proper delivery of healthcare. Two molecular tools are applied to authenticate abalone products. Firstly, forensically informative nucleotide sequencing (FINS) analysis based on mitochondria! 16S rDNA region of abalone products retailed in Hong Kong revealed that all sliced and one canned products matched the sequences of other gastropods but not Haliotis species, suggesting the presence of substitutions. Secondly, multiplex PCR with a Haliotis-specific primer was used to facilitate rapid and reliable exclusion of substitutes from abalone products. (C) 2011 Elsevier Ltd. All rights reserved.
All Author(s) ListChan WH, Ling KH, Shaw PC, Chiu SW, But PPH
Journal nameFood Control
Year2012
Month1
Day1
Volume Number23
Issue Number1
PublisherElsevier
Pages137 - 142
ISSN0956-7135
eISSN1873-7129
LanguagesEnglish-United Kingdom
KeywordsAbalone; Forensically informative nucleotide sequencing (FINS); Haliotis; Haliotis-specific primer; Multiplex PCR; Substitute
Web of Science Subject CategoriesFood Science & Technology; FOOD SCIENCE & TECHNOLOGY

Last updated on 2021-19-02 at 23:37