Effects of cathelicidin and its fragments on three key enzymes of HIV-1
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摘要Cathelicidins exhibit anti-HIV activity but it is not known if they reduce the activity of enzymes crucial to the life cycle of the retrovirus. It is shown in this investigation that human cathelicidin LL37 and its fragments LL13-37 and LL17-32 inhibited HIV-1 reverse transcriptase dose-dependently with an IC50 value of 15 mu M, 7 mu M, and 70 mu M respectively. The three peptides inhibited HIV-1 protease with a weak potency, achieving 20-30% inhibition at 100 mu M. The mechanism of inhibition was protein-protein interaction as revealed by surface plasmon resonance. The peptides were devoid of the ability to inhibit translocation of HIV-1 integrase, which has been labeled with green fluorescent protein, into the nucleus. The peptides did not exert toxicity on human peripheral blood mononuclear cells. (C) 2011 Elsevier Inc. All rights reserved.
著者Wong JH, Legowska A, Rolka K, Ng TB, Hui M, Cho CH, Lam WWL, Au SWN, Gu OW, Wan DCC
期刊名稱Peptides
詳細描述To ORKTS: DOI: 10.1016/j.peptides.2011.04.017
出版年份2011
月份6
日期1
卷號32
期次6
出版社Elsevier
頁次1117 - 1122
國際標準期刊號0196-9781
電子國際標準期刊號1873-5169
語言英式英語
關鍵詞Cathelicidin; Fragments; HIV reverse transcriptase; Integrase; Protease
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

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