Glial cells isolated from dorsal root ganglia express prostaglandin E-2 (EP4) and prostacyclin (IP) receptors
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AbstractIsolated cells from adult rat dorsal root ganglia (DRG) are frequently used as a model system to study responses of primary sensory neurons to nociceptor sensitizing agents such as prostaglandin E-2 and prostacyclin, which are presumed to act only on the neurons in typical mixed cell cultures. In the present study, we evaluated the expression of prostaglandin E-2 (EP4) and prostacyclin (IP) receptors in cultures of mixed DRG cells and in purified DRG glia. We show here that EP4 and IP receptor agonists stimulated adenylyl cyclase activity in both mixed DRG cells and in purified DRG glia, and that these responses were specifically inhibited by EP4 and IP receptor antagonists, respectively. The presence of ER, and IP receptors in DRG glia was further confirmed by the expression of EP4 and IP receptor immunoreactivity and mRNA. With the increasing awareness of neuron-glial interactions within intact DRG and the use of isolated DRG cells in the study of mechanisms underlying nociception, it will be essential to consider the role played by EP4 and IP receptor-expressing glial :cells when evaluating prostanoid-induced sensitization of DRG neurons. (C) 2011 Elsevier B.V. All rights reserved.
All Author(s) ListNg KY, Wong YH, Wise H
Journal nameEuropean Journal of Pharmacology
Year2011
Month7
Day1
Volume Number661
Issue Number1-3
PublisherElsevier
Pages42 - 48
ISSN0014-2999
eISSN1879-0712
LanguagesEnglish-United Kingdom
KeywordscAMP; Dorsal root ganglia; EP4 receptors; IP receptors; Prostaglandin E-2; Satellite glial cells
Web of Science Subject CategoriesPharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-05-07 at 00:14