Metergoline-induced cell death in Candida krusei
Publication in refereed journal

香港中文大學研究人員

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摘要Metergoline possesses potent antifungal activity against Candida krusei, a notorious yeast species that is inherently resistant to the common antifungal agents. In an attempt to elucidate the action mechanisms of metergoline, the present study was designed to investigate its effects on a number of classical markers of apoptosis in C. krusei. The results showed that transient exposure (2 h) to metergoline led to a massive intracellular accumulation of reactive oxygen species (ROS) and depolarization of mitochondrial membrane potential in a concentration-dependent fashion. Analyses of the treated fungal cells after prolonged incubation (12 h) with metergoline by flow cytometry and fluorescence microscopy clearly demonstrated phosphatidylserine externalization, the presence of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling-positive cells and fungal cells undergoing necrosis. Taken together, our data provide evidence that metergoline elicited cell death process in C. krusei through elevation of the intracellular ROS level and perturbation of mitochondrial homeostasis, followed by damage of nucleus and eventual cell demise. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
著者Kang K, Wong KS, Fong WP, Tsang PWK
期刊名稱Fungal Biology
出版年份2011
月份3
日期1
卷號115
期次3
出版社Elsevier
頁次302 - 309
國際標準期刊號1878-6146
電子國際標準期刊號1878-6162
語言英式英語
關鍵詞Candida krusei; DNA fragmentation; Metergoline; Mitochondrial membrane potential; Reactive oxygen species
Web of Science 學科類別Mycology; MYCOLOGY

上次更新時間 2020-18-10 於 00:14