Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)
Publication in refereed journal


引用次數
替代計量分析
.

其它資訊
摘要Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif. (C) 2010 Elsevier Inc. All rights reserved.
著者Ng YM, Yang YH, Sze KH, Zhang XA, Zheng YT, Shaw PC
期刊名稱Journal of Structural Biology
出版年份2011
月份4
日期1
卷號174
期次1
出版社Elsevier
頁次164 - 172
國際標準期刊號1047-8477
語言英式英語
關鍵詞Anti-HIV-1; Luffin P1; Nuclear magnetic resonance; Ribosome-inactivating peptide
Web of Science 學科類別Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

上次更新時間 2020-24-11 於 00:10