The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard
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AbstractThe Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.
All Author(s) ListPeng LF, Kapp EA, Fenyo D, Kwon MS, Jiang P, Wu SF, Jiang Y, Aguilar MI, Ahmed N, Baker MS, Cai ZW, Chen YJ, Van Chi P, Chung MCM, He FC, Len ACL, Liao PC, Nakamura K, Ngai SM, Paik YK, Pan TL, Poon TCW, Salekdeh GH, Simpson RJ, Sirdeshmukh R, Srisomsap C, Svasti J, Tyan YC, Dreyer FS, McLauchlan D, Rawson P, Jordan TW
Journal nameProteomics
Volume Number10
Issue Number22
PublisherWiley-VCH Verlag
Pages4142 - 4148
LanguagesEnglish-United Kingdom
KeywordsAsia Oceania Human Proteome Organisation; Cell Biology; Integral membrane proteins; Liver; Membrane proteomics
Web of Science Subject CategoriesBiochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY

Last updated on 2020-25-09 at 00:26