Nerve Growth Factor-Induced Differentiation of PC12 Cells Is Accompanied by Elevated Adenylyl Cyclase Activity
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AbstractRat pheochromocytoma (PC12) cells characteristically undergo differentiation when cultured with nerve growth factor (NGF). Here we show that NGF dramatically increased the adenylyl cyclase-activating property of forskolin in PC12 cells. This effect of NGF was well maintained even when NGF was removed after 4 days, even though the morphological features of neuronal differentiation were rapidly lost on removal of NGF. The enhanced cAMP production in response to forskolin could be due to a synergistic interaction between forskolin and endogenously released agonists acting on G(s)-coupled receptors. However, responses to forskolin were not attenuated by antagonists of adenosine A2 receptors or pituitary adenylate cyclase-activating polypeptide (PACAP) receptors, suggesting that adenosine and PACAP were not involved. Adenylyl cyclases 3, 6 and 9 were the predominant isoforms expressed in PC12 cells, but we found no evidence for NGF-induced changes in expression levels of any of the 9 adenylyl cyclase isoforms, nor in the expression of G alpha(s). These findings highlight that NGF has a subtle influence on adenylyl cyclase activity in PC12 cells which may influence more than the neurite extension process classically associated with neuronal differentiation. Copyright (C) 2010 S. Karger AG, Basel
All Author(s) ListYung HS, Lai KH, Chow KBS, Ip NY, Tsim KWK, Wong YH, Wu Z, Wise H
Journal nameNeurosignals
Detailed descriptionTo ORKTS: doi: 10.1159/000310977
Volume Number18
Issue Number1
Pages32 - 42
LanguagesEnglish-United Kingdom
KeywordsA2 receptors; cAMP; Forskolin; Nerve growth factor; PACAP receptors; PC12 cells
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY; Neurosciences; NEUROSCIENCES; Neurosciences & Neurology

Last updated on 2020-27-06 at 00:23