Differential gene expression profiling on the muscle of acetylcholinesterase knockout mice: A preliminary analysis
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AbstractAcetylcholinesterase (AChE) (EC. 3.1.1.7) is the acetylcholine-hydrolyzing enzyme that plays an essential role on cholinergic neurotransmission at the synapses of the brain and at the neuromuscular junctions. In order to gain insight into the molecular mechanisms of neuromuscular dysfunction associated with AChE deficiency, we have compared the RNA expression profiles of the muscles of AChE knockout mice with those of the wild-type siblings. Total RNA from the leg muscle of the mice of the wild-type and the AChE nullizygous mice were subjected to microarray analyses with Affymetrix GeneChip (R) Mouse Gene 1.0 ST Array. The pair-wise comparison of gene expression levels of the 28,853 mRNA transcripts showed that 303 genes were either up- or down-regulated by more than 2.0 folds in the AChE knockout mice. The interaction study of these differentially regulated genes indicated that some of these genes are clustered in biological functions that are related to lipid metabolism and the skeletal-muscular functions. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
All Author(s) ListLin HQ, Choi R, Chan KL, Ip D, Tsim KWK, Wan DCC
Name of Conference10th International Meeting on Cholinesterases
Start Date of Conference20/09/2009
End Date of Conference25/09/2009
Place of ConferenceSibenik
Journal nameChemico-Biological Interactions
Year2010
Month9
Day6
Volume Number187
Issue Number1-3
PublisherElsevier
Pages120 - 123
ISSN0009-2797
eISSN1872-7786
LanguagesEnglish-United Kingdom
KeywordsAChE deficient mice; Gene profiling; Microarray analysis
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY; Toxicology; TOXICOLOGY

Last updated on 2020-06-08 at 01:28