Immunological Study of the Effects of Dendrobium on the Exocrine Function of Eyes
Refereed conference paper presented and published in conference proceedings


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AbstractDry eye disease (DED) is a heterogeneous disorder of the ocular surface in which the common denominator is chronic inflammation-related immune dysregulation. This inflammatory mechanism prompts the maturation of antigen presenting cells (APCs) such as resident dendritic cells (DC) in the ocular tissue, subsequently attract the infiltration of T helper cells to the ocular tissues 1,2. Several Dendrobium (石斛) species are used for the purpose of being beneficial to the stomach and promoting the production of body fluid and clearing heat. Taken together, the inhibitory effect of Dendrobium on DC maturation and T cell activity and DED-related inflammatory mediators may offer a novel approach in DED therapy.
Different components from the extracts may be responsible for the inhibitory and stimulatory activities. The ethyl acetate part of Dendrobium loddigesii Rolfe美花石斛 could significantly suppress cytokine production (IL-1β, IL-10 and TNF-α) from lipopolysaccharide (LPS)-activated peripheral blood mononuclear cells (PBMCs)/DCs and LPS/leukotriene (LT)B4-treated PBMCs in a dose-dependent way. The ethanol extract of Dendrobium loddigesii Rolfe美花石斛 shows the same tendency but less effective. The ethanol extract of Dendrobium loddigesii Rolfe美花石斛 under 250 μg/mL concentration could significantly induce inflammatory cytokine production (IL-1β) from non-activated PBMCs, while the ethyl acetate part shows the opposite effect. The ethanol extract of Dendrobium officinale铁皮石斛 at 250 and 500 μg/mL could significantly induce cytokine production (IL-1β) from non-activated PBMCs. The inhibitory effect of Dendrobium on DC maturation and DED related inflammatory mediators may offer a novel approach in DED therapy. Further sub-fractionation of the美花石斛extract is suggested to clarify this issue.
All Author(s) ListJia Wei LING, Chung Lap CHAN, Ping Chung LEUNG, Chun Kwok WONG
Name of Conference16th International Postgraduate Symposium on Chinese Medicine
Start Date of Conference13/08/2020
End Date of Conference14/08/2020
Place of ConferenceOnline
Country/Region of ConferenceHong Kong
Year2020
LanguagesEnglish-United Kingdom

Last updated on 2021-06-12 at 10:55