Ethyl Acetate Fraction of the Root of Rubia cordifolia L. Inhibits Keratinocyte Proliferation In Vitro and Promotes Keratinocyte Differentiation In Vivo: Potential Application for Psoriasis Treatment
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AbstractPsoriasis is a skin disease associated with hyperproliferation and aberrant differentiation of keratinocytes. Our previous studies have identified the root of Rubia cordifolia L. as a potent antiproliferative and apoptogenic agent in cultured HaCaT cells (IC(50) 1.4 mu g/ml). In the present study, ethanolic extract of Radix Rubiae was fractioned sequentially with hexane, ethyl acetate (EA), n-butanol and water. EA fraction was found to possess most potent antiproliferative action on HaCaT cells (IC(50) 0.9 mu g/ml. Mechanistic study revealed that EA fraction induced apoptosis on HaCaT cells, as it was capable of inducing apoptotic morphological changes. Annexin V-PI staining assay also demonstrated that EA fraction significantly augmented HaCaT apoptosis. In addition, EA fraction decreased mitochondrial membrane potential in a concentration- and time-dependent manner. The standardized EA fraction was formulated into topical gel and its keratinocyte-modulating action was tested on mouse tail model. EA fraction dose-dependently increased the number and thickness of granular layer and epidermal thickness on mouse tail skin, indicative of the keratinocyte differentiation-inducing activity. Taking the in vitro and in vivo findings together, the present preclinical study confirms that EA fraction is a promising antipsoriatic agent warranting further development for psoriasis treatment. Copyright (C) 2009 John Wiley & Sons, Ltd.
All Author(s) ListLin ZX, Jiao BW, Che CT, Zuo Z, Mok CF, Zhao M, Ho WKK, Tse WP, Lam KY, Fan RQ, Yang ZJ, Cheng CHK
Journal namePhytotherapy Research
Volume Number24
Issue Number7
PublisherWiley: 12 months
Pages1056 - 1064
LanguagesEnglish-United Kingdom
Keywordsapoptosis; differentiation; HaCaT keratinocytes; mouse tail test; proliferation; psoriasis; Rubia cordifolia L.
Web of Science Subject CategoriesChemistry, Medicinal; CHEMISTRY, MEDICINAL; Pharmacology & Pharmacy; PHARMACOLOGY & PHARMACY

Last updated on 2020-06-08 at 01:23