Schizolysin, a hemolysin from the split gill mushroom Schizophyllum commune
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AbstractA monomeric hemolysin with a molecular mass of 29 kDa was isolated from fresh fruiting bodies of the split gill mushroom Schizophyllum commune. The hemolysin was purified by successive adsorption on DEAE-cellulose, carboxymethyl-cellulose and Q-Sepharose and finally gel filtration on Superdex 75. This demonstrated the N-terminal sequence ATNYNKCPGA, different from those of previously reported fungal and bacterial hemolysins. The hemolysin was stable up to 40 degrees C. Only partial activity remained at 50 and 60 degrees C. Activity was indiscernible at 70 degrees C. A pH of 6.0 was optimal for activity. The hemolytic activity was most potently inhibited by dithiothreitol, sucrose and raffinose, followed by cellobiose, maltose, rhamnose, inulin, lactose, fructose and inositol. The metal ions Cu2+, Mg2+, Zn2+, Al3+ and Fe3+ significantly, and Pb2+ to a lesser extent, curtailed the activity of the hemolysin. The hemolysin inhibited HIV-1 reverse transcriptase with an IC(50) of 1.8 mu M.
All Author(s) ListHan CH, Zhang GQ, Wang HX, Ng TB
Journal nameFEMS Microbiology Letters
Detailed descriptionTo ORKTS: doi: 10.1111/j.1574-6968.2010.02022.x
Year2010
Month8
Day1
Volume Number309
Issue Number2
PublisherOxford University Press (OUP): Policy B - Oxford Open Option D
Pages115 - 121
ISSN0378-1097
LanguagesEnglish-United Kingdom
Keywordshemolysin; mushroom; purification; Schizophyllum commune
Web of Science Subject CategoriesMicrobiology; MICROBIOLOGY

Last updated on 2021-25-02 at 00:45