Schizolysin, a hemolysin from the split gill mushroom Schizophyllum commune
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香港中文大學研究人員

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摘要A monomeric hemolysin with a molecular mass of 29 kDa was isolated from fresh fruiting bodies of the split gill mushroom Schizophyllum commune. The hemolysin was purified by successive adsorption on DEAE-cellulose, carboxymethyl-cellulose and Q-Sepharose and finally gel filtration on Superdex 75. This demonstrated the N-terminal sequence ATNYNKCPGA, different from those of previously reported fungal and bacterial hemolysins. The hemolysin was stable up to 40 degrees C. Only partial activity remained at 50 and 60 degrees C. Activity was indiscernible at 70 degrees C. A pH of 6.0 was optimal for activity. The hemolytic activity was most potently inhibited by dithiothreitol, sucrose and raffinose, followed by cellobiose, maltose, rhamnose, inulin, lactose, fructose and inositol. The metal ions Cu2+, Mg2+, Zn2+, Al3+ and Fe3+ significantly, and Pb2+ to a lesser extent, curtailed the activity of the hemolysin. The hemolysin inhibited HIV-1 reverse transcriptase with an IC(50) of 1.8 mu M.
著者Han CH, Zhang GQ, Wang HX, Ng TB
期刊名稱FEMS Microbiology Letters
詳細描述To ORKTS: doi: 10.1111/j.1574-6968.2010.02022.x
出版年份2010
月份8
日期1
卷號309
期次2
出版社Oxford University Press (OUP): Policy B - Oxford Open Option D
頁次115 - 121
國際標準期刊號0378-1097
語言英式英語
關鍵詞hemolysin; mushroom; purification; Schizophyllum commune
Web of Science 學科類別Microbiology; MICROBIOLOGY

上次更新時間 2021-15-04 於 23:43