Abnormal proliferation and differentiation of osteoblasts from girls with adolescent idiopathic scoliosis to melatonin
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摘要Melatonin deficiency has been postulated as an etiologic factors in adolescent idiopathic scoliosis (AIS). In previous studies, melatonin was shown to regulate skeletal growth and bone formation in both humans and rats. Although it remains controversial whether there are differences in serum melatonin level between AIS and control subjects, melatonin signaling pathway dysfunction in osteoblasts has been reported in patients with AIS. Recently, our group found that melatonin receptor 1B (MT2) gene polymorphism was associated with the occurrence of AIS. Hence, the present study investigated the effect of melatonin on AIS osteoblasts. In vitro assays were performed with osteoblasts isolated from 17 severe AIS girls and nine control subjects. The osteoblasts were exposed to different concentrations of melatonin for 3 days. The effects of melatonin on cell proliferation (as evidenced by MTT assay) and differentiation (demonstrated by alkaline phosphatase activity) were determined. In the control group, melatonin significantly stimulated osteoblasts to proliferate and differentiate. However, in the AIS group, the stimulatory effects of melatonin were not discernible. Importantly, this finding demonstrated that there is a significant difference between AIS and control osteoblasts in functional response toward melatonin. Melatonin-stimulated proliferation of control osteoblasts was inhibited by the MT2 antagonist, 4-phenyl-2-propionamidotetraline, as well as by luzindole, a nonselective melatonin receptor antagonist, suggesting that MT2 is associated with the proliferative action of melatonin. The lack of response in AIS osteoblasts might be because of dysfunction of the melatonin signaling pathway, which may contribute to the low bone mineral density and abnormal skeletal growth observed in patients with AIS.
著者Man GCW, Wang WWJ, Yeung BHY, Lee SKM, Ng BKA, Hung WY, Wong JH, Ng TB, Qiu Y, Cheng JCY
期刊名稱Journal of Pineal Research
詳細描述To ORKTS: doi: 10.1111/j.1600-079X.2010.00768.x
出版年份2010
月份8
日期1
卷號49
期次1
出版社Wiley: 12 months
頁次69 - 77
國際標準期刊號0742-3098
電子國際標準期刊號1600-079X
語言英式英語
關鍵詞adolescent; differentiation; idiopathic scoliosis; melatonin; osteoblast; proliferation
Web of Science 學科類別Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM; Neurosciences; NEUROSCIENCES; Neurosciences & Neurology; Physiology; PHYSIOLOGY

上次更新時間 2020-20-10 於 00:48