2,3,7,8-Tetrachlorodibenzo-para-dioxin increases aromatase (CYP19) mRNA stability in MCF-7 cells
Publication in refereed journal

香港中文大學研究人員

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摘要Dioxins are industrial pollutants that can be bio-accumulative in our food chain. Humans can be exposed to this class of pollutant through contaminated food, air, drinking water, etc. Displaying both pro- and anti-estrogenic properties, these pollutants are also known as endocrine disruptors. The link between breast cancer and TODD exposure has not been resolved, although TCDD is classified as 'known human carcinogen'. Estrogen is a documented risk factor for breast carcinogenesis. In the present study, effect of 2,3,7,8-tetrachlorodibenzo-para-dioxin (TCDD) on estrogen synthesis was investigated in the breast cancer cells MCF-7. Our results showed that TCDD increased the aromatase activity in a time- and dose-dependent manner. Real-time PCR and western blot analysis verified the induced expression by 2-3-fold; however, gene reporter assay revealed that the promoter activity of exons 1.3 and II was not elevated. Further investigation indicated that TCDD slowed down the CYP19 mRNA degradation with concurrent activation of ERK. The ERK inhibitor U0726 could reverse the extended stability of the transcripts. In summary this study demonstrated that TCDD might induce a post-transcriptional regulatory mechanism of gene expression in breast cancer cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
著者Chan MY, Huang H, Leung LK
期刊名稱Molecular and Cellular Endocrinology
出版年份2010
月份4
日期12
卷號317
期次1-2
出版社ELSEVIER IRELAND LTD
頁次8 - 13
國際標準期刊號0303-7207
語言英式英語
關鍵詞CYE19; TCDD
Web of Science 學科類別Cell Biology; CELL BIOLOGY; Endocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

上次更新時間 2020-20-09 於 01:12