Advanced Glycation End-Products Induce Tubular CTGF via TGF-beta-Independent Smad3 Signaling
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摘要Advanced glycation end-products (AGEs) can induce expression of connective tissue growth factor (CTGF), which seems to promote the development of diabetic nephropathy, but the exact signaling mechanisms that mediate this induction are unknown. Here, AGEs induced CTGF expression in tubular epithelial cells (TECs) that either lacked the TGF-beta 1 gene or expressed dominant TGF-beta receptor II, demonstrating independence of TGF-beta. Furthermore, conditional knockout of the gene encoding TGF-beta receptor II from the kidney did not prevent AGE-induced renal expression of CTGF and collagen I. More specific, AGEs induced CTGF expression via the receptor for AGEs-extracellular signal-regulated kinase (RAGE-ERK)/p38 mitogen-activated protein kinase-Smad cross-talk pathway because inhibition of this pathway by several methods (anti-RAGE antibody, specific inhibitors, or dominant negative adenovirus to ERK1/2 and p38) blocked this induction. Overexpressing Smad7 abolished AGE-induced Smad3 phosphorylation and CTGF expression, demonstrating the necessity for activation of Smad signaling in this process. More important, knockdown of either Smad3 or Smad2 demonstrated that Smad3 but not Smad2 is essential for CTGF induction in response to AGEs. In conclusion, AGEs induce tubular CTGF expression via the TGF-beta-independent RAGE-ERK/p38-Smad3 cross-talk pathway. These data suggest that overexpression of Smad7 or targeting Smad3 may have therapeutic potential for diabetic nephropathy.
著者Chung ACK, Zhang HY, Kong YZ, Tan JJ, Huang XR, Kopp JB, Lan HY
期刊名稱Journal of the American Society of Nephrology
出版年份2010
月份2
日期1
卷號21
期次2
出版社American Society of Nephrology
頁次249 - 260
國際標準期刊號1046-6673
電子國際標準期刊號1533-3450
語言英式英語
Web of Science 學科類別Urology & Nephrology; UROLOGY & NEPHROLOGY

上次更新時間 2021-10-01 於 23:40