Activation of the black seabream (Acanthopagrus schlegeli) somatolactin-alpha gene promoter by Pit-1c in the Hepa-T1 cell-line
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AbstractSomatolactin (SL) is a pituitary hormone of the growth hormone (GH) gene family found only in fish. To understand the regulation of this hormone at the level of gene transcription, we obtained a SL alpha gene from black seabream (bsb), with its 5' flanking promoter region carrying several putative transcription factors including seven binding sites for pituitary-specific transcription factor 1 (Pit-1). To study the actions of Pit-1 on this gene promoter, we cloned three variants of bsbPit-1 (Pit-1a, Pit-1b and Pit-1c) derived from alternative splicing of mRNA or differential transcription start sites from black seabream pituitary. The deduced amino acid sequences of these Pit-1s contained 371 amino acids (aa), 333 and 311 aa for the three Pit-1 variants, Pit-la, Pit-1b and Pit-1c, respectively, with diverse regions of Pit-1 located at the transactivation domain. The actions of bsbPit-1 variants on the bsbSL gene promoter were investigated using a co-transfection assay, with a reporter gene using a transient expression assay in Hepa-T1 cells. The N-terminus truncated isoform bsbPit-1c showed the highest level of activity on SL alpha gene promoter activation in Hepa-T1 cells; however, neither Pit-la nor Pit-1b activated the bsbSL gene promoter in the same study. (C) 2009 Elsevier Inc. All rights reserved.
All Author(s) ListTian J, Chan KM
Journal nameGeneral and Comparative Endocrinology
Volume Number166
Issue Number1
Pages186 - 199
LanguagesEnglish-United Kingdom
KeywordsAlternative splicing; Pit-1 variants; Somatolactin-alpha gene; Transcription factors
Web of Science Subject CategoriesEndocrinology & Metabolism; ENDOCRINOLOGY & METABOLISM

Last updated on 2020-07-08 at 00:18