Multiple gene dysfunctions lead to high cancer-susceptibility: evidences from a whole-exome sequencing study
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摘要A total of $275 million has been launched to The Cancer Genome Atlas Project for genomic mapping of more than 20 types of cancers. The major challenge is to develop high throughput and cost-effective techniques for human genome sequencing. We developed a targeted exome sequencing technology to routinely determine human exome sequence. As a proof-of-concept, we chose a unique patient, who underwent three high mortalities cancers, i.e., breast, gallbladder and lung cancers, to reveal the genetic cause of high-cancer-susceptibility. Total 24,545 SNPs were detected. 10,868 (44.27%) SNPs were within coding regions, and 1,077 (4.38%) located in the UTRs. 3367 genes were hit by 4480 non-sysnonymous mutations in CDS with truncation of 30 proteins; and 10 mutations occurred at the splice sites that would generate different protein isoforms. Substitutions or premature terminations occurred in 132 proteins encoded by cancer-associated genes. CARD8 was completely loss; ANAPC1 was pre-translationally terminated from the transcripts of one allele. On the Ras-MAPK pathway, 18 genes were homozygously mutated. 15 growth factors/cytokines and their receptors, 9 transcription factors, 6 proteins on WNT signaling pathway, and 16 cell surface and extracellular proteins may be dysfunctioned. Exome sequencing made it possible for individualized cancer therapy.
著者He ML, Chen Y, Chen Q, He YQ, Zhao J, Wang J, Yang HM, Kung HF
期刊名稱American Journal of Cancer Research
出版年份2011
月份1
日期1
卷號1
期次4
出版社e-Century Publishing
頁次562 - 573
國際標準期刊號2156-6976
語言英式英語
關鍵詞cancer; genetic variations; high-cancer-susceptibility; whole-exome sequencing
Web of Science 學科類別Oncology

上次更新時間 2020-27-11 於 00:11