Authentication of Hedyotis products by adaptor ligation-mediated PCR and metabarcoding
Publication in refereed journal

替代計量分析
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其它資訊
摘要DNA barcoding is a widely used tool for species identification and authentication. However, it may not be applicable to highly processed herbal products due to severe DNA fragmentation. The emergence of DNA metabarcoding provides an alternative way to solve the problem. In this study, we are the first to combine the use of adaptor ligation-mediated PCR method and metabarcoding to reveal species identities in herbal products. As an illustration, we applied the method on three Hedyotis herbal products collected from China and Thailand. Results showed that H. diffusa and H. corymbosa were present in the products which were consistent with their label claims. Our study indicated that the adaptor ligation-mediated PCR with metabarcoding approach is useful for authentication of highly processed herbal products.
著者Mavis Hong-Yu Yik, Yat-Tung Lo, Xiao Lin, Wei Sun, Ting-Fung Chan, Pang-Chui Shaw
期刊名稱Journal of Pharmaceutical and Biomedical Analysis
出版年份2021
月份3
日期20
卷號196
文章號碼113920
國際標準期刊號0731-7085
電子國際標準期刊號1873-264X
語言美式英語

上次更新時間 2021-18-09 於 23:53