Combination of magnesium ions and Vitamin C alleviates synovitis and osteophyte formation in osteoarthritis of mice
Publication in policy or professional journal

替代計量分析
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其它資訊
摘要Introduction
We previously demonstrated that magnesium ions (Mg2+) was a novel therapeutic alternative for osteoarthritis (OA) through promoting the hypoxia inducible factor-1α (HIF-1α)-mediated cartilage matrix synthesis. However, oxidative stress can inhibit the expression of HIF-1α, amplify the inflammation that potentially impairs the therapeutic efficacy of Mg2+ in OA. Vitamin (VC), a potent antioxidant, may enhance the efficacy of Mg2+ in OA treatment. This study aims to investigate the efficacy of combination of Mg2+ and VC on alleviating joint destruction and pain in OA.

Material and methods
Anterior cruciate ligament transection with partial medial meniscectomy induced mice OA model were randomly received intra-articular injection of either saline, MgCl2 (0.5 mol/L), VC (3 mg/ml) or MgCl2 (0.5 mol/L) plus VC (3 mg/ml) at week 2 post-operation, twice weekly, for 2 weeks. Joint pain and pathological changes were assessed by gait analysis, histology, western blotting and micro-CT.

Results
Mg2+ and VC showed additive effects to significantly alleviate the joint destruction and pain. The efficacy of this combined therapy could sustain for 3 months after the last injection. We demonstrated that VC enhanced the promotive effect of Mg2+ on HIF-1α expression in cartilage. Additionally, combination of Mg2+ and VC markedly promoted the M2 polarization of macrophages in synovium. Furthermore, combination of Mg2+ and VC inhibited osteophyte formation and expressions of pain-related neuropeptides.

Conclusions
Intra-articular administration of Mg2+ and VC additively alleviates joint destruction and pain in OA. Our current formulation may be a cost-effective alternative treatment for OA.
出版社接受日期21.10.2020
著者Hao Yao, Jiankun Xu, Jiali Wang, Yifeng Zhang, Nianye Zheng, Jiang Yue, Jie Mi, Lizhen Zheng, Bingyang Dai, Wenhan Huang, Shuhang Yung, Peijie Hu, Yechun Ruan, Qingyun Xue, Kiwai Ho, Ling Qin
期刊名稱Bioactive Materials
出版年份2021
月份5
卷號6
期次5
出版社Elsevier
頁次1341 - 1352
電子國際標準期刊號2452-199X
語言美式英語
關鍵詞OsteoarthritisMagnesiumVitamin CCartilageInflammationOsteophyte

上次更新時間 2021-23-10 於 00:09