Optimised tissue clearing minimises distortion and destruction during tissue delipidation
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Aims
A variety of tissue clearing techniques have been developed to render intact tissue transparent. For thicker samples, additional partial tissue delipidation is required before immersion into the final refractive index (RI)-matching solution, which alone is often inadequate to achieve full tissue transparency. However, it is difficult to determine a sufficient degree of tissue delipidation, excess of which can result in tissue distortion and protein loss. Here, we aim to develop a clearing strategy that allows better monitoring and more precise determination of delipidation progress.

Methods
We combined the detergent sodium dodecyl sulphate (SDS) with OPTIClear, a RI-matching solution, to form a strategy termed Accurate delipidation with Optimal Clearing (Accu-OptiClearing). Accu-OptiClearing allows for a better preview of the final tissue transparency achieved when immersed in OPTIClear alone just before imaging. We assessed for the changes in clearing rate, protein loss, degree of tissue distortion, and preservation of antigens.

Results
Partial delipidation using Accu-OptiClearing accelerated tissue clearing and better preserved tissue structure and antigens than delipidation with SDS alone. Despite achieving similar transparency in the final OPTIClear solution, more lipids were retained in samples cleared with Accu-OptiClearing compared to SDS.

Conclusions
Combining the RI-matching solution OPTIClear with detergents, Accu-OptiClearing, can avoid excessive delipidation, leading to accelerated tissue clearing, less tissue damage and better preserved antigens.
出版社接受日期26.10.2020
著者Krit Lee, Hei Ming Lai, Maja Hoejvang Soerensen, Edward Sai-Kam Hui, Victor Wan-San Ma, William Chi-Shing Cho, Yuen-Shan Ho, Raymond Chuen-Chung Chang
期刊名稱Neuropathology and Applied Neurobiology
出版年份2021
月份4
卷號47
期次3
出版社Wiley
頁次441 - 453
國際標準期刊號0305-1846
語言美式英語

上次更新時間 2021-23-11 於 23:55