rad21 Is Involved in Corneal Stroma Development by Regulating Neural Crest Migration
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AbstractPreviously, we identified RAD21R450C from a peripheral sclerocornea pedigree. Injection of this rad21 variantmRNAintoXenopus laevis embryos disrupted the organization of corneal stroma fibrils. To understand the mechanisms of RAD21-mediated corneal stroma defects, gene expression and chromosome conformation analysis were performed using cells from family members affected by peripheral sclerocornea. Both gene expression and chromosome conformation of cell adhesion genes were aected in cells carrying the heterozygous rad21 variant. Since cell migration is essential in early embryonic development and sclerocornea is a congenital disease, we studied neural crest migration during cornea development in X. laevis embryos. In X. laevis embryos injected with rad21 mutant mRNA, neural crest migration was disrupted, and the number of neural crest-derived periocular mesenchymes decreased significantly in the corneal stroma region. Our data indicate that the RAD21R450C variant contributes to peripheral sclerocornea by modifying chromosome conformation and gene expression, therefore disturbing neural crest cell migration, which suggests RAD21 plays a key role in corneal stroma development.
Acceptance Date21/10/2020
All Author(s) ListBi Ning Zhang, Yu Liu, Qichen Yang, Pui Ying Leung, Chengdong Wang, Thomas Chi Bun Wong, Clement C. Tham, Sun On Chan, Chi Pui Pang, Li Jia Chen, Job Dekker, Hui Zhao, Wai Kit Chu
Journal nameInternational Journal of Molecular Sciences
Volume Number21
Issue Number20
Article number7807
LanguagesEnglish-United Kingdom
Keywordsrad21, neural crest migration, Xenopus laevis, corneal stroma

Last updated on 2021-16-06 at 00:12