Maturation of Nasopharyngeal and Oral Microbiome in Chinese Infants
Refereed conference paper presented and published in conference proceedings


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AbstractRATIONALE: Early-life evolution of mucosal microbiome is related to maturation of host immunity. This prospective study characterized changes in microbiota at nasopharynx and oral mucosa in Chinese infants.
METHODS: 119 full-term newborns (51.3% males) of Chinese parents with average allergy risk were followed at 1, 6 and 12 months to contribute a total of 297 nasopharyngeal (NP) and oral swabs. Microbiome at these sites were characterized by 16S rRNA sequencing using Illumina Miseq PE300. Taxonomic identification was based on SILVA 138 (99%) database and analyzed by QIIME2 v.2020.2.
RESULTS: Oral cavity had more diverse microbial composition than nasopharynx (jclrj > 2.0 by ANCOM). Shannon biodiversity index (SBI) was higher in oral than NP microbiome (median 3.99 versus 2.29; P<0.001). SBI of oral microbiome increased with time (P<0.001) while that of NP remained stable (P50.186). At single timepoints, NP microbiome was represented by Corynebacterium and Dolosigranulum while oral microbiome were represented by Streptococcus, Gemella, and Rothia. Staphylococcus (31.8% at 1-month to 2.43% at 12-month) was replaced by Dolosigranulum (7.73% at 1-month to 31.7% at 12-month) and Moraxella (2.29% at 1-month to 16.5% at 12-month) in nasopharynx. NP microbiome had declining abundance of Actinobacteria, while Bacteroidetes and Fusobacteria increased and Firmicutes decreased over time in oral cavity.
CONCLUSIONS: Oral microbiome is more diverse than that at nasopharynx. Corynebacterium is the most abundant microbe in nasopharynx during infancy, with the second changing from Staphylococcus to Dolosigranulum and Moraxella. Oral microbiome is dominated by Streptococcus.
Acceptance Date01/02/2021
All Author(s) ListTing Fan Leung, Yu Ping Song, Yehao Chen, Shuk Man Li, Kate Ching Ching Chan, Agnes Sze Yin Leung, Brian Hei Long Tong, Apple Chung Man Yeung, Nam Sze Cheng, Cecily Woon Wa Leung, Mei Kam Chang, Wing Hung Tam, Zigui Chen, Paul Kay Sheung Chan
Name of ConferenceAmerican Academy of Allergy Asthma and Immunology 2021 Annual Meeting
Start Date of Conference26/02/2021
End Date of Conference01/03/2021
Place of ConferenceVirtual
Country/Region of ConferenceUnited States of America
Proceedings TitleJournal of Allergy and Clinical Immunology
Year2021
Month2
Volume Number147
Issue NumberSuppl 2
PublisherAmerican Academy of Allergy Asthma and Immunology
Place of PublicationUnited States of America
PagesAB238 - AB238
ISSN0091-6749
LanguagesEnglish-United States
KeywordsBirth cohort, maturation, microbiome, upper airway

Last updated on 2021-11-06 at 00:16