Biopatterning of pluripotent stem cell derived cardiomyocytes in tissue-mimicking micro-scaffold
Refereed conference paper presented and published in conference proceedings


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AbstractNative tissue mimicry is a hallmark of regenerative medicine and an increasingly important area of research. Hitherto, there have been many attempts at regeneration of various tissue types, including cartilage, skeletal muscle and bone. Multiple strategies have been used for the regeneration of these tissue types with varying success, of which the most prominent is 3-dimensional bioprinting. While such approaches are able to recapitulate macro-scale shape and features, they have had limited success in controlling the micro-scale reconstruction of tissue morphology for the purpose of tissue regeneration. In this study, we demonstrated a novel micro-scaffold based technique for the generation of biopatterned cardiomyocytes from murine or human pluripotent stem cells (PSCs). Micro-scaffold arrays were created on biocompatible hydrogels via a combination of photo- and soft-lithography. PSCs were then seeded on top of the micro-scaffold array and differentiated in-situ. RT-qPCR analysis revealed a downregulation of pluripotent markers OCT-4, Nanog and Sox2. Concurrently, the expression of cardiac specific marker genes alpha-MHC and Myocardin was observed increased. Immunofluorescent staining depicted the downregulation of Sox-2 and the upregulation of beta-adrenergic receptor level. Taken together, the PSCs were successfully differentiated and committed to the cardiomyocytic lineage. Moreover, seeded PSCs were patterned into 50μm wide longitudinal strands mimicking native muscle fibre morphology, providing a patterning resolution of PSCs down to 50μm, which far exceed the practical limit of resolution via extrusion bioprinting of approximately 200μm. In addition, as demonstrated by the tube formation assay of HUVEC cells immuno-stained with the endothelial marker CD-31, the micro-scaffold patterned cells were aligned in a longitudinal manner when compared to the nonpatterned counterpart. These results signal the potential of the proposed micro-scaffold based biopatterning for capillarised myocardial tissue regeneration.
Acceptance Date10/12/2019
All Author(s) ListHon Son Ooi, Tak Keung Pang, Qinru Xiao, Fengjie Zhang, Wing Pui Tsang, Yi-Ping Ho, Chao Wan
Name of Conference2019 Guangzhou-Hong Kong Postgraduate Research Exchange and Symposium on Regenerative Medicine
Start Date of Conference10/12/2019
End Date of Conference11/12/2019
Place of ConferenceGuangzhou
Country/Region of ConferenceChina
Year2019
Month12
LanguagesEnglish-United Kingdom
KeywordsBiopatterning; pluripotent stem cells; cardiomyocytes

Last updated on 2020-26-11 at 20:14