MicroRNA-200 family governs ovarian inclusion cyst formation and mode of ovarian cancer spread
Publication in refereed journal

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其它資訊
摘要Epidemiologic and histopathologic findings and the laying hen model support the long-standing incessant ovulation hypothesis and cortical inclusion cyst involvement in sporadic ovarian cancer development. MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. Herewith, we show that ovarian surface epithelial (OSE) cells with ectopic miR-200 expression formed stabilized cysts in three-dimensional (3D) organotypic culture with E-cadherin fragment expression and steroid hormone pathway activation, whereas ovarian cancer 3D cultures with miR-200 knockdown showed elevated TGF-beta expression, mitotic spindle disorientation, increased lumenization, disruption of ROCK-mediated myosin II phosphorylation, and SRC signaling, which led to histotype-dependent loss of collective movement in tumor spread. Gene expression profiling revealed that epithelial-mesenchymal transition and hypoxia were the top enriched gene sets regulated by miR-200 in both OSE and ovarian cancer cells. The molecular changes uncovered by the in vitro studies were verified in both human and laying hen ovarian cysts and tumor specimens. As miR-200 is also essential for ovulation, our results of estrogen pathway activation in miR-200-expressing OSE cells add another intriguing link between incessant ovulation and ovarian carcinogenesis.
出版社接受日期09.03.2020
著者Pui-Wah CHOI, Wai Wing SO, Junzheng YANG, Shubai LIU, Ka Kui TONG, Kin Ming KWAN, Jamie S.-L. KWOK, Stephen K.W. TSUI, Shu Kay NG, Karen H. HALES, Dale B. HALES, William R. WELCH, Christopher P. CRUM, Wing-Ping FONG, Ross S. BERKOWITZ, Shu Wing NG
期刊名稱Oncogene,Oncogene
出版年份2020
月份5
卷號39
期次20
出版社NATURE PUBLISHING GROUP
頁次4045 - 4060
國際標準期刊號0950-9232
電子國際標準期刊號1476-5594
語言英式英語
Web of Science 學科類別Biochemistry & Molecular Biology;Oncology;Cell Biology;Genetics & Heredity;Biochemistry & Molecular Biology;Oncology;Cell Biology;Genetics & Heredity

上次更新時間 2020-20-10 於 00:30