Probing Chinese Herbal Formula as an Anti-Atopic Dermatitis Agent: Evaluation in Vivo and in Vitro
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摘要Atopic dermatitis (AD) is a common skin disorder characterized as erythema, eruption, lichenification and pruritus. Up to date, 2-10% of adults and 15-30% of children are suffering from AD. In the theory of traditional Chinese medicine, AD was caused by the invasion of wind, dampness and heat to sensitive body. Prof Lin’s empirical herbal formula (SZF) which contains Rehmanniae Radix, Scutellariae Radix, Phellodendri Chinensis Cortex, Dictamni Cortex and other five herbal medicines has been used for the treatment of AD in clinics for many years and is effective in relieving patients’ AD symptoms. In this study, we aimed to investigate the anti-AD effects of SZF and elucidate its underlying molecular mechanisms using in vivo and in vitro models of AD. Methods: High-performance liquid chromatography analysis were performed to control the quality of SZF extracts. AD-like skin lesions in female BALB/c mice were induced by 2,4-dinitochlorobenzene (DNCB). SZE (3.15, 6.30 and 9.45 g/kg) and dexamethasone (5mg/kg) were daily administered by gavage for 15 days. The body weight, skin thickness, skin dermatitis severity and scratching behaviors were recorded throughout the study. Histological analysis, reverse transcription-quantitative polymerase chain reaction, western blotting and ELISA analysis were used to illuminate the molecular targets associated with anti-AD effects of SZF. The anti-inflammatory effect of SZF was investigated through evaluating the change of nitric oxide (NO), chemokines and pro-/inflammatory cytokines in lipopolysaccharide (LPS) stimulated RAW264.7 cells. Results: SZF contained baicalin (4.92%), berberine (2.90%), paeoniflorin (0.26%) and phillyrin (0.10%). In vivo study, SZF could significantly alleviate AD-like skin lesions and decrease dorsal skin thickening in DNCB-treated mice. DNCB induced scratching behaviors obviously reduced in SZF-treated mice. The anti-pruritus effect of SZF was better than dexamethasone. Infiltration of mast cell and epidermal thickening in dorsal skin and ears were also inhibited by SZF treatment. Additionally, levels of AD-specific markers as IgE, histamine, interleukin (IL)-4, thymic stromal lymphopoietin (TSLP) in serum obviously decreased in SZF-treated mice. SZF reduced both the concentration and mRNA expression of T-helper (Th)-2 cell cytokines IL-4, IL-6, IL-13 and IL-31 levels but increased Th-1 cell cytokine IFN- γ level in the skin of DNCB-treated mice. SZF might also contribute to improvement of skin barrier function through up-regulating the protein expression lever of filaggrin in the skin of DNCB-treated mice. In vitro study, SZF (400ug/ml) also inhibited the secretions of NO, chemokines (granulocyte-macrophage colony stimulating factor and monocyte chemoattractant protein-1) and pro-/inflammatory cytokines (TNF-α, PGE2, interferon- γ, IL-1β, IL-2, IL-4 and IL-6) induced LPS in RAW264.7 cells. Conclusion: SZF might exert therapeutic effect on DNCB induced AD-like skin lesions in mice through regulation of the Th1/Th2 balance and inhibition of inflammation.

Acknowledgement:
This work was supported by grants from Li Dak Sum Yip Yio Chun R & D Centre for Chinese Medicine, The Chinese University of Hong Kong (Project No.: 5501816).
著者Lan Wang, Yan-Fang Xian, Siu Po Ip, Zhi-Xiu Lin, Justin Che Yuen Wu
會議名稱The 15th International Postgraduate Symposium on Chinese Medicine (IPSCM)
會議開始日16.08.2019
會議完結日16.08.2019
會議地點Hong Kong
會議國家/地區香港
出版年份2019
月份8
語言英式英語

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