Doping control analysis of total arsenic in equine plasma
Publication in refereed journal


摘要Arsenic can be easily found in our surrounding environment. Due to its ubiquitous nature, horse urine and blood invariably contain low levels of arsenic. Nevertheless, inorganic arsenic is an effective doping agent due to its ability to induce gastroenteritis. The misuse of arsenic in horseracing has been controlled by an international urinary threshold of total arsenic at 0.3 µg/mL. With the increasing popularity of using blood as a matrix for doping control testing in the past decade, an equivalent threshold for plasma total arsenic is highly desirable. In this paper, an inductively coupled plasma-mass spectrometry method has been developed for quantifying total arsenic in equine plasma. Statistical analysis determined that the data from a population study of 1552 post-race and out-of-competition plasma samples fits a Gaussian mixture model with two Gaussian components. A rounded-up provisional threshold for plasma total arsenic at 2.5 ng/mL was subsequently established. Results from administration trials with a sodium arsanilate-containing supplement showed that both urinary and plasma arsenic were significantly elevated after administration. The maximum urinary detection time was around 22 hours based on the international threshold. However, the maximum plasma detection time would be longer than 73 hours if the provisional threshold of 2.5 ng/mL adopted. In view of the high discrepancy between the urine and plasma detection times, a revised plasma threshold of 15 ng/mL is proposed to afford a comparable detection time in both matrices. The risk of a normal sample exceeding the proposed plasma total arsenic threshold is practically zero.
著者George H. M. Chan, Wenlu Tang, Peter Curl, Yuanyuan Lin, Terence S. M. Wan, Emmie N. M. Ho
期刊名稱Drug Testing and Analysis
關鍵詞arsenic, horse, inductively coupled plasma-mass spectrometry, plasma, threshold

上次更新時間 2020-22-11 於 23:45