Intra-articular injection of magnesium chloride attenuates osteoarthritis progression in rats
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AbstractObjective
To explore the effects of Mg2+ on the expression of osteoarthritic markers in human cartilage and synovium tissue explants. To investigate the therapeutic effect of intra-articular injection of Mg2+ in an established rat OA (Osteoarthritis) model of anterior cruciate ligament transection with partial medial meniscectomy (ACLT + PMM).

Design
Human cartilage and synovium explants were collected from total knee replacement surgeries and incubated with MgCl2 (20 mmol/L) in vitro. A rat OA model was established by ACLT + PMM surgery in 450–500 g male Sprague Dawley (SD) rats. To select the optimal dose, intra-articular injections of MgCl2 (0.05, 0.5, 5 mol/L) were performed at 4 weeks after the surgery every 3 days for 2 weeks. The effect of optimized MgCl2 was further determined by histology, immunohistochemistry, and quantitative real-time polymerase chain reaction.

Results
The expressions of osteoarthritic markers in human cartilage and synovium explants were inhibited by Mg2+ in vitro. Immunohistochemical analysis further suggested the inhibitory effects of Mg2+ on the expression of MMP-13 and IL-6 in the human tissue explants. Cartilage degeneration and synovitis in ACLT + PMM rats were significantly improved by intra-articular injections of Mg2+ (0.5 mol/L). Immunohistochemical analysis also showed the regulatory effects of Mg2+ on osteoarthritic markers in both cartilage and synovium in rats, consistent with in vitro results.

Conclusion
Intra-articular injections of Mg2+ at 0.5 mol/L attenuate the progression of OA in the ACLT + PMM rat model. Such effect was at least in part explained by the promotion of cartilage matrix synthesis and the suppression of synovial inflammation.
Acceptance Date30/08/2019
All Author(s) ListH Yao, JK Xu, NY Zheng, JL Wang, SW Mok, YW Lee, L Shi, JY Wang, J Yue, SH Yung, PJ Hu, YC Ruan, YF Zhang, KW Ho, L Qin
Journal nameOsteoarthritis and Cartilage
Year2019
Month12
Volume Number27
Issue Number12
PublisherElsevier
Pages1811 - 1821
ISSN1063-4584
eISSN1522-9653
LanguagesEnglish-United States
KeywordsMagnesium, Osteoarthritis, Cartilage

Last updated on 2024-21-08 at 00:36