Discovery of G-quadruplex-forming sequences in SARS-CoV-2
Publication in refereed journal

香港中文大學研究人員
替代計量分析
.

其它資訊
摘要The outbreak caused by the novel coronavirus SARS-CoV-2 has been declared a global health emergency. G-quadruplex structures in genomes have long been considered essential for regulating a number of biological processes in a plethora of organisms. We have analyzed and identified 25 four contiguous GG runs (G2NxG2NyG2NzG2) in the SARS-CoV-2 RNA genome, suggesting putative G-quadruplex-forming sequences (PQSs). Detailed analysis of SARS-CoV-2 PQSs revealed their locations in the open reading frames of ORF1 ab, spike (S), ORF3a, membrane (M) and nucleocapsid (N) genes. Identical PQSs were also found in the other members of the Coronaviridae family. The top-ranked PQSs at positions 13385 and 24268 were confirmed to form RNA G-quadruplex structures in vitro by multiple spectroscopic assays. Furthermore, their direct interactions with viral helicase (nsp13) were determined by microscale thermophoresis. Molecular docking model suggests that nsp13 distorts the G-quadruplex structure by allowing the guanine bases to be flipped away from the guanine quartet planes. Targeting viral helicase and G-quadruplex structure represents an attractive approach for potentially inhibiting the SARS-CoV-2 virus.
出版社接受日期12.05.2020
著者Ji D, Juhas M, Tsang CM, Kwok CK, Li Y, Zhang Y
期刊名稱Briefings in Bioinformatics
出版年份2021
月份3
卷號22
期次2
出版社Oxford University Press
頁次1150 - 1160
國際標準期刊號1467-5463
電子國際標準期刊號1477-4054
語言英式英語

上次更新時間 2021-18-09 於 23:49