Psoralen prevents bone loss in ovariectomized mice through suppressing NFATc1-mediated osteoclast differentiation
Refereed conference paper presented and published in conference proceedings

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AbstractOsteoporosis is the most common metabolic bone disease characterized by a decrease in bone mass and deterioration in bone microarchitecture, leading to bone fragility and high risk of fractures. Discovery of novel therapies remains a significant clinical demand. In recent years, attention is paid to traditional Chinese medicine (TCM) based therapies for osteoporosis.Psoralen (PS), a coumarin derivative compound extracted from Psoralea croylifolia L., a widely prescribed herb in TCM formulas, is shown to possess osteoprotective effect. However,
the underlying pharmacological mechanisms of PS on regulating bone mass remain unclear. Here, we performed systemic in vitro and in vivo analyses to examine the cellular and molecular mechanisms of PS on osteoclast function as well as its therapeutic efficacy in ovariectomized (OVX) mice. Our data showed that PS inhibited bone marrow mononuclear cells (BMMNCs) derived osteoclast formation indicated by tartrate-resistant acid phosphatase (TRAP) staining without affecting cell viability. Meanwhile, PS interfered F-actin ring formation and suppressed bone resorption. PS attenuated the expression of osteoclast marker genes including nuclear factor of activated T-cells cytoplasmic1(NFATc1), TRAP, calcitonin receptor, cathepsin K, carbonic anhydrase Ⅱ , MMP9 and Fra-2. Mechanistically, PS suppressed RANKL-induced activation of Akt and the levels of transcription factors controlling osteoclast differentiation such as c-Fos, c-Jun and NFATc1. In addition, intraperitoneal delivery of PS prevented bone loss in OVX mice accompanied by decreased osteoclast numbers and the serum level of TRACP5b. Our results suggest that PS may serve as a potential agent for treatment of osteoporosis through targeting NFATc1 mediated osteoclastogenesis.
All Author(s) ListWANG Yanyan, ZHANG Fengjie, TSANG Wing Pui, WAN Chao
Name of ConferenceThe 12th Guangzhou International Conference on Stem Cell and Regenerative Medicine
Start Date of Conference18/11/2019
End Date of Conference20/11/2019
Place of ConferenceGuangzhou
Country/Region of ConferenceChina
LanguagesEnglish-United Kingdom

Last updated on 2020-02-12 at 15:13