Fecal microbial DNA markers serve for screening colorectal neoplasm in asymptomatic subjects
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Officially Accepted for Publication


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AbstractBackground
We have previously shown that fecal microbial markers might be useful for non‐invasive diagnosis of colorectal cancer (CRC) and adenoma. Here we assessed the application of microbial DNA markers, as compared to and in combination with fecal immunochemical test (FIT), in detecting CRC and adenoma in symptomatic patients and asymptomatic subjects.

Methods
We recruited 676 subjects (210 CRC, 115 advanced adenoma (AA), 86 non‐advanced adenoma and 265 non‐neoplastic controls), including 241 symptomatic and 435 asymptomatic subjects. Fecal abundances of Fusobacterium nucleatum , a Lachnoclostridium sp. m3 , Bacteroides clarus , and Clostridium hathewayi were quantified by quantitative PCR. Combining score of the four microbial markers (4Bac) and diagnostic prediction were determined using our previously established scoring model and cutoff values and FIT with a cutoff of 100ng Hb/mL.

Results
4Bac detected similar percentages of CRC [85.3% (95%CI: 79.2%‐90.2%) vs 84.9% (68.1%‐94.9%)] and AA [35.7% (12.8%‐64.9%) vs 38.6% (29.1%‐48.8%)], while FIT detected more CRC [72.1% (63.7%‐79.4%) vs 66.7% (48.2%‐82.0%)] and AA [28.6% (8.4%‐58.1%) vs 16.8% (10.1%‐25.6%)], in symptomatic vs asymptomatic subjects respectively. Focusing on the asymptomatic cohort, 4Bac was more sensitive for diagnosing CRC and AA than FIT (P <0.001), with lower specificity [83.3% (77.6%‐88.0%) vs 98.6% (96.0%‐99.7%)]. FIT failed to detect any non‐advanced adenoma [0% (0.0%‐4.2%)] compared to 4Bac [41.9% (31.3%‐53.0%), P <0.0001]. Combining 4Bac with FIT improved sensitivities for CRC [90.9% (75.7%‐98.1%)] and AA [48.5% (38.4%‐58.7%)] detection.

Conclusions
Quantitation of fecal microbial DNA markers may serve as a new test, stand‐alone or in combination with FIT, for screening colorectal neoplasm in asymptomatic subjects.
Acceptance Date03/07/2020
All Author(s) ListJessie Qiaoyi Liang, Sunny H Wong, Chun Ho Szeto, Eagle SH Chu, Harry C Lau, Yingxuan Chen, Jingyuan Fang, Jun Yu, Joseph JY Sung
Journal nameJournal of Gastroenterology and Hepatology
Year2020
PublisherWiley
ISSN0815-9319
eISSN1440-1746
LanguagesEnglish-United States

Last updated on 2020-25-10 at 01:01