Towards characterizing brain-wide neural circuitry dynamics underlying chemosensory avoidance in larval zebrafish
Refereed conference paper presented and published in conference proceedings

摘要Chemicals provide important environmental cues to guide animal behavior. While bilateral olfactory input is required for several animal species for optimizing odor navigation, the underlying circuit mediating bilateral integration of chemosensory information remains poorly understood. We employ larval zebrafish to study the bilateral integration of chemical signals upon encountering cadaverine, a diamine molecule released by decaying flesh, that drives chemosensory guided behaviour. In a customized arena where fluidic streams are spatially defined by laminar flow, we observed that 5 - 7 dpf freely swimming zebrafish larvae actively avoid the cadaverine-containing stream by increasing turn angles of subsequent swim bouts. Such behaviour necessitates bilateral olfactory input, as unilateral ablation of olfactory placode largely compromised the avoidance. Further on, we have built an integrated microfluidics - light sheet imaging platform for precise chemical stimulation while performing simultaneous neural activity and behavioural imaging, to characterize the brain-wide circuitry mediating the sensorimotor transformation.
著者Sy SKH, Chan DCW, Lai HM, Li Z, Choi JCH, Mok VCT, Ko H
會議名稱Neuroscience 2019
會議地點Chicago, IL

上次更新時間 2021-07-01 於 09:23