Antibody-free rapid diagnosis of malaria in whole blood with surface-enhanced Raman Spectroscopy using Nanostructured Gold Substrate
Publication in refereed journal

替代計量分析
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其它資訊
摘要Purpose
The aim of this study is to establish a rapid antibody-free diagnostic method of malaria infection with Plasmodium falciparum and Plasmodium vivax in whole blood with Surface-enhanced Raman Spectroscopy using Nanostructured Gold Substrate.

Materials and methods
The blood samples collected from patients were first lysed and centrifuged before dropping on the gold nano-structure (AuNS) substrate. Malaria diagnosis was performed by detecting Raman peaks from Surface Enhanced Raman Spectroscopy (SERS) with a 532 nm laser excitation.

Results
Raman peaks at 1370 cm−1, 1570 cm−1, and 1627 cm−1, known to have high specificity against interference from other mosquito-borne diseases such as Dengue and West Nile virus infection, were selected as the fingerprint markers associated with P. falciparum and P. vivax infection. The limit of detection was 10−5 dilution, corresponding to the concentration of parasitized blood cells of 100/mL. A total number of 25 clinical samples, including 5 from patients with P. falciparum infection, 10 with P. vivax infection and 10 from healthy volunteers, were evaluated to support its clinical practical use. The whole assay on malaria detection took 30 min to complete.

Conclusions
While the samples analyzed in this work have strong clinical relevance, we have clearly demonstrated that sensitive malaria detection using AuNS-SERS is a practical direction for rapid in-field diagnosis of malaria infection.
出版社接受日期15.09.2019
著者Wang W, Dong RL, Gu D, He JA, Yi P, Kong SK, Ho HP, Loo JF, Wang W, Wang Q
期刊名稱Advances in Medical Sciences
出版年份2020
月份3
卷號65
期次1
出版社Elsevier
頁次86 - 92
國際標準期刊號1896-1126
電子國際標準期刊號1898-4002
語言英式英語

上次更新時間 2020-23-11 於 23:46