BZS20 is required for chloroplast development and assembly of thylakoid protein complexes in Arabidopsis
Refereed conference paper presented and published in conference proceedings


摘要Chloroplasts are crucial organelles for photosynthesis and plant survival; however, the detailed mechanisms of chloroplast development remain not fully understood. From a genetic mutant screen, we identified a novel albino mutant bzs20 in Arabidopsis, which showed seedling lethality and has severe defects in chloroplast development. Gene cloning uncovered that the mutant phenotypes were caused by the disruption of a nuclear gene, BZS20, which encodes a novel integral thylakoid membrane protein with unknown function. BZS20 harbors an uncharacterized function domain, DUF1279, and is conserved across green algae and plants. Overexpression of BZS20 completely rescues the bzs20 mutant phenotypes, while knockout of the BZS20 by CRISPR-Cas9 recapitulates the mutant phenotypes. Comparative proteomics and immunoblot analyses between the mutant and wild-type plants indicated that loss of BZS20 function severely impairs the accumulation of multiprotein complexes in thylakoid membranes, particularly photosystem I, photosystem II and components of cytochrome b6f complex. Taken together, our results demonstrated that BZS20 is a novel and key component of chloroplast development, playing essential roles in the assembly or stabilization of thylakoid complexes.
著者Na Li, Wing Shing Wong, King Shing Wong, Lei Feng, Jun-Xian He
會議名稱Plant Biology 2019
會議地點San Jose, CA
關鍵詞Chloroplast development, thylakoid protein, Arabidopsis

上次更新時間 2020-15-06 於 14:12