BZS20 is required for chloroplast development and assembly of thylakoid protein complexes in Arabidopsis
Refereed conference paper presented and published in conference proceedings

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AbstractChloroplasts are crucial organelles for photosynthesis and plant survival; however, the detailed mechanisms of chloroplast development remain not fully understood. From a genetic mutant screen, we identified a novel albino mutant bzs20 in Arabidopsis, which showed seedling lethality and has severe defects in chloroplast development. Gene cloning uncovered that the mutant phenotypes were caused by the disruption of a nuclear gene, BZS20, which encodes a novel integral thylakoid membrane protein with unknown function. BZS20 harbors an uncharacterized function domain, DUF1279, and is conserved across green algae and plants. Overexpression of BZS20 completely rescues the bzs20 mutant phenotypes, while knockout of the BZS20 by CRISPR-Cas9 recapitulates the mutant phenotypes. Comparative proteomics and immunoblot analyses between the mutant and wild-type plants indicated that loss of BZS20 function severely impairs the accumulation of multiprotein complexes in thylakoid membranes, particularly photosystem I, photosystem II and components of cytochrome b6f complex. Taken together, our results demonstrated that BZS20 is a novel and key component of chloroplast development, playing essential roles in the assembly or stabilization of thylakoid complexes.
Acceptance Date01/03/2019
All Author(s) ListNa Li, Wing Shing Wong, King Shing Wong, Lei Feng, Jun-Xian He
Name of ConferencePlant Biology 2019
Start Date of Conference03/08/2019
End Date of Conference07/08/2019
Place of ConferenceSan Jose, CA
Country/Region of ConferenceUnited States of America
LanguagesEnglish-United States
KeywordsChloroplast development, thylakoid protein, Arabidopsis

Last updated on 2020-15-06 at 14:12