Quantitation of DNA reactive pyrrolic metabolites of senecionine - A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Pyrrolizidine alkaloids (PAs) are carcinogenic phytochemicals, inducing liver tumors in experimental rodents. We previously determined that (+/-)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP), 7-glutathione-DHP, 7-cysteine-DHP, 7-N-acetylcysteine-DHP, and 1-CHO-DHP are DNA reactive pyrrolic metabolites potentially associated with PA-induced liver tumor initiation. In this study, we developed an LC/MS/MS multiple reaction monitoring (MRM) mode method to identify and quantify these metabolites formed from the metabolism of senecionine, a carcinogenic PA, by mouse, rat, and human liver microsomes, and primary rat hepatocytes. Together with the chemically prepared standards of these metabolites, this represents an accurate and convenient LC/MS/MS analytical method for quantifying these five reactive pyrrolic metabolites in biological systems.
著者Qingsu XIA, Xiaobo HE, Qiang SHI, Ge LIN, Peter P. FU
期刊名稱Journal of Food and Drug Analysis
出版年份2020
月份1
卷號28
期次1
出版社Elsevier
頁次167 - 174
國際標準期刊號1021-9498
語言英式英語
關鍵詞Pyrrolizidine alkaloid, Senecionine, Pyrrolic metabolites, Carcinogenicity, LC/MS/MS analysis
Web of Science 學科類別Food Science & Technology;Pharmacology & Pharmacy;Food Science & Technology;Pharmacology & Pharmacy

上次更新時間 2021-18-09 於 00:00