Monochorionic twins with selective fetal growth restriction: insight from placental whole-transcriptome analysis
Publication in refereed journal


Times Cited
Altmetrics Information
.

Other information
AbstractBackground
The underlying pathomechanisms in placental related selective fetal growth restriction in monochorionic diamniotic twin pregnancy is not known.

Objective
To investigate any differences in placental transcriptomic profile between the selectively growth restricted twins and the normally grown co-twins in monochorionic diamniotic twin pregnancies.

Study Design
This was a prospective study of monochorionic diamniotic twin pregnancies complicated by selective fetal growth restriction. Placental biopsies were obtained from the subjects in the delivery suite. The placental transcriptome of the selectively growth restricted twin was compared to that of the normally grown co-twin. This study was divided into two stages: (1) Gene discovery phase: placental tissues from five monochorionic diamniotic twin pregnancies complicated with selective fetal growth restriction plus two control twin pregnancies underwent transcriptome profiling. Transcriptome profiling was carried out by whole genome RNA sequencing; (2) Validation phase: placental tissues from 13 monochorionic diamniotic twin pregnancies with selective fetal growth restriction underwent RNA and protein validation. RNA and protein expression level of candidate genes were determined by quantitative real-time PCR and immunohistochemistry staining.

Results
A total of 1429 transcripts were differentially expressed in placentae of selective growth restricted twin pairs, where 610 were up-regulated and 819 were down-regulated. Endoplasmic Reticulum Lectin and Mannose-6-Phosphate Receptor were consistently differentially up-regulated in all placentae of the selectively growth restricted twins. Quantitative real-time PCR and immunohistochemistry staining validated the results (p<0.05).

Conclusion
The expression of Endoplasmic Reticulum Lectin and Mannose-6-Phosphate Receptor, which are important for angiogenesis and fetal growth, are significantly increased in the placentae of the selectively growth restricted twin of a monochorionic twin pair.
Acceptance Date05/05/2020
All Author(s) ListWei Li, Claire Yik Lok Chung, Chi Chiu Wang, Ting Fung Chan, Maran Bo Wah Leung, Oi Ka Chan, Ling Wu, Kubi Appiah, Piya Chaemsaithong, Yvonne Kwun Yue Cheng, Liona Chiu Yee Poon, Tak Yeung Leung
Journal nameAmerican Journal of Obstetrics and Gynecology
Year2020
Month11
Volume Number223
Issue Number5
PublisherElsevier
Pages749.e1 - 749.e16
ISSN0002-9378
LanguagesEnglish-United States
Keywordsendoplasmic reticulum, lectin, immunohistochemistry, staining, monochorionic diamniotic twin, mannose-6-phosphate receptor, placenta, quantitative real-time PCR, RNA sequencing, selective fetal growth restriction, transcriptome

Last updated on 2021-18-06 at 00:38