Development of a sensitive DMD-based 2D SPR sensor array using single-point detection strategy for multiple aptamer screening
Publication in refereed journal

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摘要In this paper, we report a novel single-point detection strategy for 2D solid-state angular interrogated SPR (surface plasmon resonance) sensor array for multiple aptamer screening. The scheme operates with two DMDs (digital micro-mirror devices), which suffices a single-point detector for high-throughput 2D SPR measurements without sacrificing the sensitivity. After having verified the biosensing capability of the system, in terms of sensitivity and repeatability, using standard BSA-anti-BSA antibody interaction, we further conducted experiments to perform rapid screening of aptamer candidates for binding with tumor necrosis factor-α (TNF-α). The selected anti-TNF-α aptamers were subsequently evaluated as an in vitro cell protection agent against TNF-α-induced cell death. Our experiments confirm that the anti-TNF-α aptamer screening approach offers high potential as an alternative to that based on antibodies for diagnostic and therapeutic applications.
出版社接受日期07.10.2019
著者Dongping Wang, Jacky Fong Chuen Loo, Wei Lin, Qiang Geng, Erika Kit Shan Ngan, Siu Kai Kong, Yeung Yam, Shih-Chi Chen, Ho Pui Ho
期刊名稱Sensors and Actuators B: Chemical
出版年份2020
月份2
卷號305
出版社Elsevier
出版地USA
文章號碼127240
國際標準期刊號0925-4005
語言美式英語
關鍵詞DMD, 2D SPR sensor, single-point detection, SPR sensor array, Single-point detector, Aptamer screening

上次更新時間 2020-22-11 於 00:03