Development of a sensitive DMD-based 2D SPR sensor array using single-point detection strategy for multiple aptamer screening
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AbstractIn this paper, we report a novel single-point detection strategy for 2D solid-state angular interrogated SPR (surface plasmon resonance) sensor array for multiple aptamer screening. The scheme operates with two DMDs (digital micro-mirror devices), which suffices a single-point detector for high-throughput 2D SPR measurements without sacrificing the sensitivity. After having verified the biosensing capability of the system, in terms of sensitivity and repeatability, using standard BSA-anti-BSA antibody interaction, we further conducted experiments to perform rapid screening of aptamer candidates for binding with tumor necrosis factor-α (TNF-α). The selected anti-TNF-α aptamers were subsequently evaluated as an in vitro cell protection agent against TNF-α-induced cell death. Our experiments confirm that the anti-TNF-α aptamer screening approach offers high potential as an alternative to that based on antibodies for diagnostic and therapeutic applications.
Acceptance Date07/10/2019
All Author(s) ListDongping Wang, Jacky Fong Chuen Loo, Wei Lin, Qiang Geng, Erika Kit Shan Ngan, Siu Kai Kong, Yeung Yam, Shih-Chi Chen, Ho Pui Ho
Journal nameSensors and Actuators B: Chemical
Volume Number305
Place of PublicationUSA
Article number127240
LanguagesEnglish-United States
KeywordsDMD, 2D SPR sensor, single-point detection, SPR sensor array, Single-point detector, Aptamer screening

Last updated on 2021-17-09 at 00:14