The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes
Publication in refereed journal

替代計量分析
.

其它資訊
摘要Zeranol is an approved but controversial growth-promoting agent for livestock in North America. It is a mycotoxin metabolite secreted by the Fusarium family fungi. The regulatory bodies in this region have established the acceptable daily intake and exposure below the level would not significantly increase the health risk for humans. However, their European counterparts have yet to establish an acceptable level and do not permit the use of this agent in farm animals. Given the growth-promoting ability of zeranol, its effect on energy metabolism was investigated in the current study. Our results indicated that zeranol could induce glucose transporter type 4 (GLUT4) expression in 3T3 L1 cells at 10 μM and initiate the translocation of the glucose transporter to the membrane as assayed by confocal microscopy. The translocation was likely triggered by the increase of GLUT4 and p-Akt. The insulin signal transduction pathway of glucose translocation was analyzed by Western blot analysis. Since no increase in the phosphorylated insulin receptor substrate in zeranol-treated cells was evidenced, the increased p-Akt and GLUT4 amount should be the mechanism dictating the GLUT4 translocation. In summary, this study showed that zeranol could perturb glucose metabolism in differentiated 3T3 L1 adipocytes. Determining the growth-promoting mechanism is crucial to uncover an accepted alternative to the general public.
出版社接受日期08.04.2020
著者Yan Qin Tan, Qing Li, Lin Wang, Leo C. Chiu-Leung, Lai K. Leung
期刊名稱Chemosphere
出版年份2020
月份8
卷號253
出版社Elsevier
文章號碼126772
國際標準期刊號0045-6535
電子國際標準期刊號1879-1298
語言美式英語
關鍵詞Zeranol, GLUT4, adipocytes

上次更新時間 2020-20-09 於 00:59