Adipose-derived stem cells and cancer cells fuse to generate cancer stem cell-like cells with increased tumorigenicity
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AbstractAdipose-derived stem cells (ADSCs) are a type of mesenchymal stem cells isolated from adipose tissue and have the ability to differentiate into adipogenic, osteogenic and chondrogenic lineages. Despite their great therapeutic potentials, previous studies showed that ADSCs could enhance the proliferation and metastatic potential of breast cancer cells (BCCs). In this study, we found that ADSCs fused with BCCs spontaneously, while breast cancer stem cell (CSC) markers CD44+CD24-/lowEpCAM+ were enriched in this fusion population. We further assessed the fusion hybrid by multicolor DNA FISH and mouse xenograft assays. Only single nucleus was observed in the fusion hybrid, confirming that it was a synkaryon. In vivo mouse xenograft assay indicated that the tumorigenic potential of the fusion hybrid was significantly higher than that of the parent tumorigenic triple-negative BCC line MDA-MB-231. We had compared the fusion efficiency between two BCC lines, the CD44-rich MDA-MB-231 and the CD44-poor MCF-7, with ADSCs. Interestingly, we found that the fusion efficiency was much higher between MDA-MB-231 and ADSCs, suggesting a potential mechanism of cell fusion may lie in the dissimilarity between these two cell lines. The cell fusion efficiency was hampered by knocking down the CD44. Altogether, our findings suggest that CD44-mediated cell fusion could be a potential mechanism for generating CSCs.
Acceptance Date09/01/2020
All Author(s) ListYuk Wah CHAN, Chun SO, Ka Long YAU, Kung Chun CHIU, Xiya WANG, Franky Leung CHAN, Suk Ying TSANG
Journal nameJournal of Cellular Physiology
Year2020
Month10
Volume Number235
Issue Number10
PublisherWiley
Pages6794 - 6807
ISSN0021-9541
eISSN1097-4652
LanguagesEnglish-United States

Last updated on 2021-21-01 at 23:50