An Ability of Programin 2 to Reverse Senescence of Human Bone Marrow Derived Mesenchymal Stem Cells
Refereed conference paper presented and published in conference proceedings


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AbstractHuman mesenchymal stem cells (MSCs) are multipotent connective tissue cells that are one of the most attractive stem cell source for tissue engineering and cell therapy. MSCs possess a potential to self-renew and differentiate into various types of cells such as osteoclasts, adipocytes, chondrocytes and neurons. Compared to the pluripotent stem cells these cells are also easier to harvest and free from ethical complications. MSCs have also good immunosuppressive capacity and they do not form teratomas in vivo. MSCs proliferate well in vitro but old patients’ MSCs enter senescence a lot faster compared to the young patients’ MSCs. This limits their use in tissue engineering and therapy since old patients are usually in higher demand of treatments. Therefore, it is crucial to enhance the proliferation capacity of these cells. In this project we tested an ability of the small molecule Programin 2 to reverse the senescence of bone marrow derived mesenchymal stem cells (BM-MSCs). Level of various proliferation markers such as phosphorylated histone H3 (PH3) and senescence associated beta-galactosidase staining were observed. In addition, an expression of proliferation associated genes such as Ki67 and SIRT1 and the level of senescence associated genes such as p21 and p16 were investigated after the Programin 2 treatment. Also the morphological changes were detected after the treatment. The results of this project have showed evidences that small molecule Programin 2 has potential to re-activate the proliferation in senescent BM-MSCs.
All Author(s) ListGrete LAANE, Paulisally Hau Yi LO, Kenneth Ka Ho LEE
Name of Conference2019 ISSCR/KSSCR International Symposium
Start Date of Conference26/09/2019
End Date of Conference27/09/2019
Place of ConferenceSeoul
Country/Region of ConferenceSouth Korea
Year2019
Month9
LanguagesEnglish-United States

Last updated on 2020-01-06 at 10:01