Age-related Changes of Bone Quality and Osteocyte Activity in Male C57BL/6J Mice
Refereed conference paper presented and published in conference proceedings


摘要Introduction: This study aimed to examine age-related bone quality changes in C57BL/6J mice and to investigate whether these changes are associated with osteocyte activity.
Methods: Micro-computed tomography was used to measure trabecular and cortical architecture in the right femur and tibia of the mice between age 1 and 12 months. Osteocyte mRNA markers in bone tissues was determined by quantitative polymerase chain reaction.
Results: Bone mineral density and bone volume density of trabecular bone were greatest at age 1 month and declined steadily thereafter. Trabecular bone loss was characterised by decreased trabecular number, increased trabecular spacing, and decreased connectivity. In contrast, trabecular bone thickness increased to a maximum value at age 6 months and declined slowly thereafter. Cortical bone thickness increased from age 3 to 6 months and was maintained or slightly decreased thereafter. Cortical bone area of the distal femur increased from age 3 to 12 months and then declined. The cortical bone area of the midshaft cortical bone increased to a maximum value at age 6 months. Stiffness and failure load of the cortical bone peaked at age 6 months and then declined. The mRNA level of osteocyte markers such as Dmp1, E11, and SOST increased from age 3 to 6 months and declined thereafter.
Conclusions: The age-related changes in bone quality in C57BL/6J male mice were similar to those found during human ageing. Similar pattern of change was observed in osteocyte particularly in cortical bone. The C57BL/6J mice maybe an appropriate surrogate to study age-related bone loss.
著者QQ Li, CKL Cheng, TP Lam, JCY Cheng, WYW Lee
會議名稱Hong Kong Orthopaedic Association 39th Annual Congress 2019 (HKOA 2019)
會議地點Hong Kong
出版地Hong Kong

上次更新時間 2020-29-05 於 11:07