Technological Advances in Multiscale Analysis of Single Cells in Biomedicine
Publication in refereed journal

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其它資訊
摘要Single-cell analysis has recently received significant attention in biomedicine. With the advances in super-resolution microscopy, fluorescence labeling, and nanoscale biosensing, new information may be obtained for the design of cancer diagnosis and therapeutic interventions. The discovery of cellular heterogeneity further stresses the importance of single-cell analysis to improve our understanding of disease mechanism and to develop new strategies for disease treatment. To this end, many studies are exploited at the single-cell level for high throughput, highly parallel, and quantitative analysis. Technically, microfluidics are also designed to facilitate single-cell isolation and enrichment for downstream detection and manipulation in a robust, sensitive, and automated manner. Further achievements are made possible by consolidating optically label-free, electrical, and molecular sensing techniques. Moreover, these technologies are coupled with computing algorithms for high throughput and automated quantitative analysis with a short turnaround time. To reflect on how the technological developments have advanced single-cell analysis, this mini-review is aimed to offer readers an introduction to single-cell analysis with a brief historical development and the recent progresses that have enabled multiscale analysis of single-cells in the last decade. The challenges and future trends are also discussed with the view to inspire forthcoming technical developments.
著者Loo JFC, Ho HP, Kong SK, Wang TH, Ho YP
期刊名稱Advanced Biosystems
出版年份2019
月份11
卷號3
期次11
出版社WILEY-V C H VERLAG GMBH
文章號碼1900138
國際標準期刊號2366-7478
語言英式英語
關鍵詞biosensing, disease diagnosis, heterogeneity, microfluidics, plasmonics
Web of Science 學科類別Materials Science, Biomaterials;Materials Science

上次更新時間 2020-21-11 於 23:53