Detection of Human Pegivirus in Blood of Immunocompromised Children with Acute Myeloid Leukemia
Refereed conference paper presented and published in conference proceedings


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AbstractObjective
To characterize blood virome in immunocompromised children by using metagenomic next-generation sequencing (mNGS).

Methods
Serial blood samples were collected from immunocompromised children with acute myeloid leukemia (AML). Blood viromes were characterized by mNGS on Illumina’s NextSeq 500 platform, followed by taxonomic classification of sequencing reads on Taxonomer. Presence of virus was validated and quantified by virus-specific RT-qPCR assays.

Results
A total of 92 blood samples collected from 20 children with AML were studied. The median age was 8 years (interquartile range [IQR]: 3-14 years). A median of 1,039,023 reads (IQR: 692,903-1,770,395 reads) were generated from each sample. mNGS detected human pegivirus (HPgV) in one sample, in which 4.2% of reads corresponded to the virus. Subsequent HPgV-specific RT-qPCR assay identified the virus in three samples from two more cases. Altogether, three (15%) cases carried HPgV. RT-qPCR Ct values ranged from 29.2 to 43.1. In one child with 16 serial samples, HPgV detection coincided with a peak cytomegalovirus level in blood.

Discussion
HPgV is commonly present at low level in blood of immunocompromised children with AML The source and relevance of HPgV in clinical management of AML remain to be determined. mNGS is useful when screening clinical samples for viruses but has a lower analytical sensitivity than molecular virus-specific RT-qPCR assays.

Acknowledgements
The study was supported in part by a research grant from the commissioned Health and Medical Research Fund of Food and Health Bureau of the HKSAR Government (to M.C.W.C.; reference number CU-17-B7).
All Author(s) ListMartin Chi-Wai Chan, Kirran N. Mohammad, Lin-Yao Zhang, Wai-Ling Lam, Frankie W.T. Cheng, Paul K.S. Chan
Name of Conference67th Annual Meeting of the Japanese Society for Virology
Start Date of Conference29/10/2019
End Date of Conference31/10/2019
Place of ConferenceTokyo
Country/Region of ConferenceJapan
Year2019
Month10
LanguagesEnglish-United Kingdom
Keywordspegivirus

Last updated on 2020-29-05 at 10:01